Natural protein ATPase inhibitor from Candida utilis mitochondria binding properties of the radiolabeled inhibitor
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چکیده
منابع مشابه
The calcium-binding ATPase inhibitor protein from bovine heart mitochondria. Purification and properties.
Two ATPase inhibitor proteins were isolated together from bovine heart mitochondria by a new procedure; each was purified further. The one inhibitor is a Ca2+-binding protein. It was found to contain 2 cysteine residues/mol as well as threonine and proline residues, all of which the other inhibitor (first isolated by Pullman and Monroy (Pullman, M.E., and Monroy, G. C. (1963) J. Biol. Chem. 238...
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An ATPase inhibitor protein was isolated from mitochondria of rat skeletal muscle by alkaline extract ion and then was purified, It differed in definit ive ways from the ATPase inhibitor protein isolated previously by Ca2§ of s u b m i t o c h o n d r i a l particles of rat s ke l e t a l muscle. The two ATPase inhibitor proteins w e r e shown to be p r e s e n t t o g e t h e r in i n t a c t ...
متن کاملBinding of the Inhibitor Protein IF1 to Bovine F1-ATPase
In the structure of bovine F(1)-ATPase inhibited with residues 1-60 of the bovine inhibitor protein IF(1), the α-helical inhibitor interacts with five of the nine subunits of F(1)-ATPase. In order to understand the contributions of individual amino acid residues to this complex binding mode, N-terminal deletions and point mutations have been introduced, and the binding properties of each mutant...
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Candida utilis contains an inducible enzyme, urea amidolyase, which catalyzes the decomposition of urea with formation of carbon dioxide and ammonia. This reaction is accompanied by the cleavage of ATP with liberation of equimolar amounts of ADP and inorganic phosphate, is strictly dependent for activity on divalent (Mg++ or Mn++) and monovalent (K+, Rb+, Csf, or NH4+) cations, and requires cat...
متن کاملPurification and properties of the urea amidolyase from Candida utilis.
Urea amidolyase was purified to homogeneity from extracts of Candida utilis. The purification involves protamine sulfate precipitation, ammonium sulfate precipitation, polyethylene glycol precipitation, Sepharose 6B gel filtration, DEAE-cellulose column chromatography, and hydroxylapatite column chromatography. The final preparation is pure as judged by disc-gel electrophoresis. The molecular w...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1977
ISSN: 0014-5793
DOI: 10.1016/0014-5793(77)81072-7